Coding
Part:BBa_K2354012:Design
Designed by: CHAN , WEI-JEN Group: iGEM17_NTHU_Taiwan (2017-10-21)
Seq Modification :
-Switch codon to avoid EcoRI, XbaI, SpeI, PstI, SacI, NheI, BglI, BamHI, NotI
-Add His tag x6 for protein purification purpose.
-Switch codon to add SacI ( Split the gene to be less than 1KB ).
-Add BamHI at 3’ terminal to facilitate protein fusion.
-Add 3bps at biobrick prefix and subfix to promote cutting effectness.